The DNA Cleanup Buffers segmentation by application delineates four primary use‑cases that together drive the market’s revenue composition. PCR purification remains the largest segment, accounting for roughly 40 % of sales, because high‑throughput laboratories require rapid, reproducible removal of primers, nucleotides, and polymerases to enable downstream quantification. Next‑Generation Sequencing (NGS) library preparation follows with an estimated 35 % share; the stringent purity demands of library construction amplify buffer consumption, especially in Illumina and Oxford Nanopore workflows. Cloning and plasmid preparation contributes about 15 % as academic and biotech facilities routinely clean up restriction digests and ligation products, where buffer cost sensitivity is higher. The residual 10 % falls under Other applications, encompassing forensic DNA recovery, diagnostic assay development, and environmental DNA extraction, each with niche but growing demand. By quantifying these application‑specific volumes, analysts can attribute proportional revenue to each sub‑market, facilitating targeted forecasting and resource allocation across the DNA Cleanup Buffers market.